Remediation Technology Summit


Phil Dennis
Phil Dennis

Senior Manager
SiREM

Phil Dennis has over 25 years of experience working in the fields of molecular biology, microbiology and environmental remediation. Phil holds a Masters of Applied Science in Civil Engineering from the University of Toronto and an Honors B.Sc. in Molecular Biology and Genetics from the University of Guelph. As a Senior Manager at SiREM, an industry leader in bioaugmentation and bioremediation testing, Phil focuses on research and development, technical marketing and sales and management of molecular genetic testing services.

PLATFORM PRESENTATION
Bioaugmentation for Anaerobic BTEX Degradation

Benzene, toluene, ethylbenzene and xylene (BTEX) are often recalcitrant under the anaerobic conditions found in many hydrocarbon contaminated aquifers. Benzene is particularly persistent and is a regulatory driver at many sites. This presentation highlights findings from a three-year project which focused on:

  1. Scale-up a benzene degrading bioaugmentation culture;
  2. Development of molecular tools to track key benzene degrading microorganisms; and
  3. Testing the culture’s effectiveness in laboratory treatability studies.

Anaerobic treatability studies were conducted using materials from numerous petroleum hydrocarbon sites. Degradation of BTEX was monitored with and without bioaugmentation and under varying electron acceptor conditions. Bioaugmentation generally accelerated benzene degradation and increases in the abundance of a benzene degrading organism (ORM-2) were observed after bioaugmentation. The benzene carboxylase gene (abcA) was also corelated with benzene degradation under nitrate reducing conditions. Successful culture scale-up, treatability studies and molecular tools have laid the foundation for upcoming field pilot tests.


Back to Platform Presenters Page